› 2j86

X-ray diffraction
3.05Å resolution

Structural analysis of the PP2C Family Phosphatase tPphA of Thermosynechococcus elongatus

Released:
DOI: 10.2210/pdb2j86/pdb
PDB entry 2j86 coloured by chain, front view.
PDB entry 2j86 coloured by chain, side view.
PDB entry 2j86 coloured by chain, top view.
Source organism: Synechococcus elongatus
Primary publication:
Structural analysis of the PP2C phosphatase tPphA from Thermosynechococcus elongatus: a flexible flap subdomain controls access to the catalytic site.
Schlicker C, Fokina O, Kloft N, Grüne T, Becker S, Sheldrick GM, Forchhammer K
J Mol Biol 376 570-81 (2008)
PMID: 18164312

Function and Biology Details

Reaction catalysed: 
[a protein]-serine/threonine phosphate + H(2)O = [a protein]-serine/threonine + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-184051 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
protein-serine/threonine phosphatase Chains: A, B
Molecule details ›
Chains: A, B
Length: 240 amino acids
Theoretical weight: 26.74 KDa
Source organism: Synechococcus elongatus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8DGS1 (Residues: 1-240; Coverage: 100%)
Gene name: tlr2243
Sequence domains: Protein phosphatase 2C
Structure domains: PPM-type phosphatase domain

Ligands and Environments

1 bound ligand:
Ligand MG 6 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P41212
Unit cell:
a: 113.29Å b: 113.29Å c: 88.61Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.24 0.24 0.295
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

9 review citations

Serine/threonine phosphatases: mechanism through structure.
Shi Y. (2009)
8 more

3 mentions without citation

Eukaryote-like serine/threonine kinases and phosphatases in bacteria.
Pereira et al. (2011)
2 more