› 2j8q

X-ray diffraction
2.3Å resolution

Crystal structure of human cleavage and polyadenylation specificity factor 5 (CPSF5) in complex with a sulphate ion.

Released:
DOI: 10.2210/pdb2j8q/pdb
PDB entry 2j8q coloured by chain, front view.
PDB entry 2j8q coloured by chain, side view.
PDB entry 2j8q coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The crystal structure of human cleavage and polyadenylation specific factor-5 reveals a dimeric Nudix protein with a conserved catalytic site.
Trésaugues L, Stenmark P, Schüler H, Flodin S, Welin M, Nyman T, Hammarström M, Moche M, Gräslund S, Nordlund P
Proteins 73 1047-52 (2008)
PMID: 18767156

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-129133 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cleavage and polyadenylation specificity factor subunit 5 Chains: A, B
Molecule details ›
Chains: A, B
Length: 227 amino acids
Theoretical weight: 26.42 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O43809 (Residues: 20-227; Coverage: 92%)
Gene names: CFIM25, CPSF25, CPSF5, NUDT21
Sequence domains: Nucleotide hydrolase
Structure domains: Nucleoside Triphosphate Pyrophosphohydrolase

Ligands and Environments

1 bound ligand:
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: P41212
Unit cell:
a: 86.4Å b: 86.4Å c: 139.7Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.22 0.218 0.269
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

The structure of human cleavage factor I(m) hints at functions beyond UGUA-specific RNA binding: a role in alternative polyadenylation and a potential link to 5' capping and splicing.
Yang et al. (2011)
5 other articles

6 mentions without citation

Delineating the structural blueprint of the pre-mRNA 3'-end processing machinery.
Xiang et al. (2014)
5 more