› 2j8s

X-ray diffraction
2.54Å resolution

Drug Export Pathway of Multidrug Exporter AcrB Revealed by DARPin Inhibitors

Released:
DOI: 10.2210/pdb2j8s/pdb
PDB entry 2j8s coloured by chain, front view.
PDB entry 2j8s coloured by chain, side view.
PDB entry 2j8s coloured by chain, top view.
Source organisms:
Primary publication:
Drug export pathway of multidrug exporter AcrB revealed by DARPin inhibitors.
Sennhauser G, Amstutz P, Briand C, Storchenegger O, Grütter MG
PLoS Biol 5 e7 (2007)
PMID: 17194213

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151928 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Multidrug efflux pump subunit AcrB Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 1055 amino acids
Theoretical weight: 114.49 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P31224 (Residues: 1-1049; Coverage: 100%)
Gene names: JW0451, acrB, acrE, b0462
Sequence domains: AcrB/AcrD/AcrF family
Structure domains:
DARPIN Chains: D, E
Molecule details ›
Chains: D, E
Length: 169 amino acids
Theoretical weight: 18.32 KDa
Source organism: synthetic construct
Expression system: Escherichia coli
Structure domains: Ankyrin repeat-containing domain

Ligands and Environments

2 bound ligands:
Ligand LMT 10 x LMT
Ligand LMU 1 x LMU
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P212121
Unit cell:
a: 146.18Å b: 157.41Å c: 246.04Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.229 0.229 0.271
Expression systems:
  • Escherichia coli BL21(DE3)
  • Escherichia coli

Quick links

Citations

55 review citations

Multidrug resistance in bacteria.
Nikaido H. (2009)
54 more

20 mentions without citation

Broad-specificity efflux pumps and their role in multidrug resistance of Gram-negative bacteria.
Nikaido et al. (2012)
19 more