› 2jaa

X-ray diffraction
3.1Å resolution

SeMet substituted Shigella Flexneri Ipad

Released:
DOI: 10.2210/pdb2jaa/pdb
PDB entry 2jaa coloured by chain, front view.
PDB entry 2jaa coloured by chain, side view.
PDB entry 2jaa coloured by chain, top view.
Source organism: Shigella flexneri
Primary publication:
Self-chaperoning of the type III secretion system needle tip proteins IpaD and BipD.
Johnson S, Roversi P, Espina M, Olive A, Deane JE, Birket S, Field T, Picking WD, Blocker AJ, Galyov EE, Picking WL, Lea SM
J Biol Chem 282 4035-44 (2007)
PMID: 17077085

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148256 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Invasin IpaD Chains: A, B
Molecule details ›
Chains: A, B
Length: 212 amino acids
Theoretical weight: 23.74 KDa
Source organism: Shigella flexneri
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P18013 (Residues: 121-332; Coverage: 64%)
Gene names: CP0126, ipaD
Sequence domains: Type III secretion systems tip complex components
Structure domains: IpaD-like

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 10 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: C2
Unit cell:
a: 137.838Å b: 44.484Å c: 100.31Å
α: 90° β: 107.96° γ: 90°
R-values:
R R work R free
0.263 0.262 0.276
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

34 review citations

Molecular pathogenesis of Shigella spp.: controlling host cell signaling, invasion, and death by type III secretion.
Schroeder et al. (2008)
33 more