› 2jac

X-ray diffraction
2.02Å resolution

Glutaredoxin Grx1p C30S mutant from yeast

Released:
DOI: 10.2210/pdb2jac/pdb
PDB entry 2jac coloured by chain, front view.
PDB entry 2jac coloured by chain, side view.
PDB entry 2jac coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Structure of glutaredoxin Grx1p C30S mutant from yeast.
Håkansson KO, Winther JR
Acta Crystallogr D Biol Crystallogr 63 288-94 (2007)
PMID: 17327665

Function and Biology Details

Reactions catalysed: 
2 glutathione + H(2)O(2) = glutathione disulfide + 2 H(2)O
RX + glutathione = HX + R-S-glutathione
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150452 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glutaredoxin-1 Chain: A
Molecule details ›
Chain: A
Length: 110 amino acids
Theoretical weight: 12.38 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P25373 (Residues: 1-110; Coverage: 100%)
Gene names: GRX1, YCL035C, YCL35C
Sequence domains: Glutaredoxin
Structure domains: Glutaredoxin

Ligands and Environments


Cofactor: Ligand GSH 1 x GSH
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MAX II BEAMLINE I711
Spacegroup: C2221
Unit cell:
a: 32.023Å b: 78.461Å c: 100.114Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.208 0.208 0.243
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

6 review citations

Fluorescent protein-based redox probes.
Meyer et al. (2010)
5 more

3 mentions without citation

Structure-function relationship of the chloroplastic glutaredoxin S12 with an atypical WCSYS active site.
Couturier et al. (2009)
2 more