› 2jad

X-ray diffraction
2.7Å resolution

Yellow fluorescent protein - glutaredoxin fusion protein

Released:
DOI: 10.2210/pdb2jad/pdb
PDB entry 2jad coloured by chain, front view.
PDB entry 2jad coloured by chain, side view.
PDB entry 2jad coloured by chain, top view.
Source organisms:
Primary publication:
Structure of glutaredoxin Grx1p C30S mutant from yeast.
Håkansson KO, Winther JR
Acta Crystallogr D Biol Crystallogr 63 288-94 (2007)
PMID: 17327665

Function and Biology Details

Reactions catalysed: 
2 glutathione + H(2)O(2) = glutathione disulfide + 2 H(2)O
RX + glutathione = HX + R-S-glutathione
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-238731 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glutaredoxin-1; Green fluorescent protein Chain: A
Molecule details ›
Chain: A
Length: 362 amino acids
Theoretical weight: 40.98 KDa
Source organisms: Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P42212 (Residues: 1-238; Coverage: 100%)
  • Canonical: P25373 (Residues: 1-110; Coverage: 100%)
Gene names: GFP, GRX1, YCL035C, YCL35C
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand SO4 2 x SO4
1 modified residue:
Ligand PIA 1 x PIA

Experiments and Validation Details

Entry percentile scores
X-ray source: MAX II BEAMLINE I711
Spacegroup: P64
Unit cell:
a: 132.111Å b: 132.111Å c: 58.722Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.214 0.214 0.247
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

6 review citations

Fluorescent protein-based redox probes.
Meyer et al. (2010)
5 more

1 mention without citation

SSMap: a new UniProt-PDB mapping resource for the curation of structural-related information in the UniProt/Swiss-Prot Knowledgebase.
David et al. (2008)