› 2jag

X-ray diffraction
1.93Å resolution

L1-intermediate of halorhodopsin T203V

Released:
DOI: 10.2210/pdb2jag/pdb
PDB entry 2jag coloured by chain, front view.
PDB entry 2jag coloured by chain, side view.
PDB entry 2jag coloured by chain, top view.
Source organism: Halobacterium salinarum R1
Primary publication:
The crystal structure of the L1 intermediate of halorhodopsin at 1.9 angstroms resolution.
Gmelin W, Zeth K, Efremov R, Heberle J, Tittor J, Oesterhelt D
Photochem Photobiol 83 369-77 (2007)
PMID: 17117890

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-109190 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Halorhodopsin Chain: A
Molecule details ›
Chain: A
Length: 274 amino acids
Theoretical weight: 28.86 KDa
Source organism: Halobacterium salinarum R1
Expression system: Halobacterium salinarum R1
UniProt:
  • Canonical: B0R2U4 (Residues: 1-274; Coverage: 100%)
Gene names: OE_1299R, hop
Sequence domains: Bacteriorhodopsin-like protein
Structure domains: Rhodopsin 7-helix transmembrane proteins

Ligands and Environments

4 bound ligands:
Ligand CL 2 x CL
Ligand PLM 1 x PLM
Ligand BOG 1 x BOG
Ligand RET 1 x RET
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: R32
Unit cell:
a: 95.042Å b: 95.042Å c: 157.674Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.237 0.237 0.261
Expression system: Halobacterium salinarum R1

Quick links

Citations

11 citation in other articles

Crystal structure of the light-driven chloride pump halorhodopsin from Natronomonas pharaonis.
Kouyama et al. (2010)
10 more

4 mentions without citation

A comprehensive review of the lipid cubic phase or in meso method for crystallizing membrane and soluble proteins and complexes.
Caffrey M. (2015)
3 more