› 2jah

X-ray diffraction
1.8Å resolution

Biochemical and structural analysis of the Clavulanic acid dehydeogenase (CAD) from Streptomyces clavuligerus

Released:
DOI: 10.2210/pdb2jah/pdb
PDB entry 2jah coloured by chain, front view.
PDB entry 2jah coloured by chain, side view.
PDB entry 2jah coloured by chain, top view.
Source organism: Streptomyces clavuligerus
Primary publication:
Clavulanic acid dehydrogenase: structural and biochemical analysis of the final step in the biosynthesis of the beta-lactamase inhibitor clavulanic acid.
MacKenzie AK, Kershaw NJ, Hernandez H, Robinson CV, Schofield CJ, Andersson I
Biochemistry 46 1523-33 (2007)
PMID: 17279617

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Clavaldehyde dehydrogenase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 247 amino acids
Theoretical weight: 26.69 KDa
Source organism: Streptomyces clavuligerus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9LCV7 (Residues: 1-247; Coverage: 100%)
Gene name: cad
Sequence domains: short chain dehydrogenase
Structure domains: NAD(P)-binding Rossmann-like Domain

Ligands and Environments

1 bound ligand:
Ligand NDP 4 x NDP
1 modified residue:
Ligand MSE 28 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-3
Spacegroup: P212121
Unit cell:
a: 58.748Å b: 122.626Å c: 126.594Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.184 0.182 0.209
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

5 review citations

The enzymes of β-lactam biosynthesis.
Hamed et al. (2013)
4 more

5 mentions without citation

Laboratory evolution of Pyrococcus furiosus alcohol dehydrogenase to improve the production of (2S,5S)-hexanediol at moderate temperatures.
Machielsen et al. (2008)
4 more