› 2jap

X-ray diffraction
2.1Å resolution

Clavulanic Acid Dehydrogenase: Structural and Biochemical Analysis of the Final Step in the Biosynthesis of the beta-Lactamase Inhibitor Clavulanic acid

Released:
DOI: 10.2210/pdb2jap/pdb
PDB entry 2jap coloured by chain, front view.
PDB entry 2jap coloured by chain, side view.
PDB entry 2jap coloured by chain, top view.
Source organism: Streptomyces clavuligerus
Primary publication:
Clavulanic acid dehydrogenase: structural and biochemical analysis of the final step in the biosynthesis of the beta-lactamase inhibitor clavulanic acid.
MacKenzie AK, Kershaw NJ, Hernandez H, Robinson CV, Schofield CJ, Andersson I
Biochemistry 46 1523-33 (2007)
PMID: 17279617

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-192260 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Clavaldehyde dehydrogenase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 247 amino acids
Theoretical weight: 26.36 KDa
Source organism: Streptomyces clavuligerus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9LCV7 (Residues: 1-247; Coverage: 100%)
Gene name: cad
Sequence domains: short chain dehydrogenase
Structure domains: NAD(P)-binding Rossmann-like Domain

Ligands and Environments

2 bound ligands:
Ligand NDP 4 x NDP
Ligand J01 4 x J01
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: P212121
Unit cell:
a: 58.076Å b: 123.423Å c: 126.78Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.186 0.184 0.216
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

5 review citations

The enzymes of β-lactam biosynthesis.
Hamed et al. (2013)
4 more

6 mentions without citation

Laboratory evolution of Pyrococcus furiosus alcohol dehydrogenase to improve the production of (2S,5S)-hexanediol at moderate temperatures.
Machielsen et al. (2008)
5 more