› 2jat

X-ray diffraction
2.6Å resolution

Structure of deoxyadenosine kinase from M.mycoides with products dcmp and a flexible dcdp bound

Released:
DOI: 10.2210/pdb2jat/pdb
PDB entry 2jat coloured by chain, front view.
PDB entry 2jat coloured by chain, side view.
PDB entry 2jat coloured by chain, top view.
Source organism: Mycoplasma mycoides subsp. mycoides SC
Primary publication:
Structure-function analysis of a bacterial deoxyadenosine kinase reveals the basis for substrate specificity.
Welin M, Wang L, Eriksson S, Eklund H
J Mol Biol 366 1615-23 (2007)
PMID: 17229440

Function and Biology Details

Reaction catalysed: 
ATP + deoxyguanosine = ADP + dGMP
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-188074 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Deoxynucleoside kinase domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 205 amino acids
Theoretical weight: 24.29 KDa
Source organism: Mycoplasma mycoides subsp. mycoides SC
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q93IG4 (Residues: 1-205; Coverage: 100%)
Gene name: dGUOK
Sequence domains: Deoxynucleoside kinase
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

3 bound ligands:
Ligand MG 2 x MG
Ligand POP 2 x POP
Ligand DCM 2 x DCM
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: P31
Unit cell:
a: 83.931Å b: 83.931Å c: 53.31Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.224 0.222 0.266
Expression system: Escherichia coli BL21(DE3)

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Citations

1 review citation

The Mysterious Multitude: Structural Perspective on the Accessory Subunits of Respiratory Complex I.
Padavannil et al. (2021)
4 other articles