› 2jb1

X-ray diffraction
1.55Å resolution

The L-amino acid oxidase from Rhodococcus opacus in complex with L- alanine

Released:
DOI: 10.2210/pdb2jb1/pdb
PDB entry 2jb1 coloured by chain, front view.
PDB entry 2jb1 coloured by chain, side view.
PDB entry 2jb1 coloured by chain, top view.
Source organism: Rhodococcus opacus
Primary publication:
The structure of a bacterial L-amino acid oxidase from Rhodococcus opacus gives new evidence for the hydride mechanism for dehydrogenation.
Faust A, Niefind K, Hummel W, Schomburg D
J Mol Biol 367 234-48 (2007)
PMID: 17234209

Function and Biology Details

Reaction catalysed: 
An L-amino acid + H(2)O + O(2) = a 2-oxo carboxylate + NH(3) + H(2)O(2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-186608 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
L-amino acid oxidase Chains: A, B
Molecule details ›
Chains: A, B
Length: 489 amino acids
Theoretical weight: 53.41 KDa
Source organism: Rhodococcus opacus
UniProt:
  • Canonical: Q8VPD4 (Residues: 46-534; Coverage: 100%)
Sequence domains: Flavin containing amine oxidoreductase
Structure domains:

Ligands and Environments


Cofactor: Ligand FAD 2 x FAD
1 bound ligand:
Ligand ALA 2 x ALA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.2
Spacegroup: P212121
Unit cell:
a: 65.9Å b: 109.929Å c: 134.487Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.145 0.143 0.167

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Citations

6 review citations

Enzymatic toxins from snake venom: structural characterization and mechanism of catalysis.
Kang et al. (2011)
5 more