› 2jdc

X-ray diffraction
1.6Å resolution

Glyphosate N-acetyltransferase bound to oxidized COA and sulfate

Released:
DOI: 10.2210/pdb2jdc/pdb
PDB entry 2jdc coloured by chain, front view.
PDB entry 2jdc coloured by chain, side view.
PDB entry 2jdc coloured by chain, top view.
Source organism: Bacillus licheniformis
Primary publication:
The molecular basis of glyphosate resistance by an optimized microbial acetyltransferase.
Siehl DL, Castle LA, Gorton R, Keenan RJ
J Biol Chem 282 11446-55 (2007)
PMID: 17272278

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-179217 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
N-acetyltransferase domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 146 amino acids
Theoretical weight: 16.62 KDa
Source organism: Bacillus licheniformis
Expression system: Escherichia coli
UniProt:
  • Canonical: Q65LG7 (Residues: 1-146; Coverage: 100%)
Gene names: BL01310, yitI
Sequence domains: Acetyltransferase (GNAT) family
Structure domains: Aminopeptidase

Ligands and Environments

2 bound ligands:
Ligand CAO 1 x CAO
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: C2
Unit cell:
a: 69.056Å b: 48.911Å c: 46.461Å
α: 90° β: 103.54° γ: 90°
R-values:
R R work R free
0.149 0.148 0.172
Expression system: Escherichia coli

Quick links

Citations

8 review citations

Synthetic biology for the directed evolution of protein biocatalysts: navigating sequence space intelligently.
Currin et al. (2015)
7 more

2 mentions without citation

The Blueprint of a Minimal Cell: MiniBacillus.
Reuß et al. (2016)
1 more