› 2jea

X-ray diffraction
2.33Å resolution

Structure of a 9-subunit archaeal exosome bound to RNA

Released:
DOI: 10.2210/pdb2jea/pdb
PDB entry 2jea coloured by chain, front view.
PDB entry 2jea coloured by chain, side view.
PDB entry 2jea coloured by chain, top view.
Source organism: Saccharolobus solfataricus
Primary publication:
RNA channelling by the archaeal exosome.
Lorentzen E, Dziembowski A, Lindner D, Seraphin B, Conti E
EMBO Rep 8 470-6 (2007)
PMID: 17380186

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero dodecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-194122 (preferred)
Entry contents:
3 distinct polypeptide molecules
1 distinct RNA molecule
Macromolecules (4 distinct):
Exosome complex component Rrp42 Chain: A
Molecule details ›
Chain: A
Length: 277 amino acids
Theoretical weight: 30.42 KDa
Source organism: Saccharolobus solfataricus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9UXC0 (Residues: 1-275; Coverage: 100%)
Gene names: C20_023, SSO0732, rrp42
Sequence domains:
Structure domains: GHMP Kinase, N-terminal domain
Exosome complex component Rrp41 Chain: B
Molecule details ›
Chain: B
Length: 250 amino acids
Theoretical weight: 27.76 KDa
Source organism: Saccharolobus solfataricus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9UXC2 (Residues: 1-248; Coverage: 100%)
Gene names: SSO0735, rrp41
Sequence domains:
Structure domains: GHMP Kinase, N-terminal domain
Exosome complex component Rrp4 Chain: I
Molecule details ›
Chain: I
Length: 251 amino acids
Theoretical weight: 28.23 KDa
Source organism: Saccharolobus solfataricus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9UXC4 (Residues: 1-249; Coverage: 100%)
Gene names: C20_026, SSO0736, rrp4
Structure domains:
RNA Chain: C
Molecule details ›
Chain: C
Length: 35 nucleotides
Theoretical weight: 11.43 KDa

Ligands and Environments

1 bound ligand:
Ligand 1PE 1 x 1PE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P213
Unit cell:
a: 135.85Å b: 135.85Å c: 135.85Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.207 0.205 0.243
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

21 review citations

Nucleases: diversity of structure, function and mechanism.
Yang W. (2011)
20 more

8 mentions without citation

Towards deciphering the principles underlying an mRNA recognition code.
Serganov et al. (2008)
7 more