› 2jeq

X-ray diffraction
1.94Å resolution

Family 5 xyloglucanase from Paenibacillus pabuli in complex with ligand

Released:
DOI: 10.2210/pdb2jeq/pdb
Model geometry
Fit model/data
PDB entry 2jeq coloured by chain, front view.
PDB entry 2jeq coloured by chain, side view.
PDB entry 2jeq coloured by chain, top view.
Source organism: Paenibacillus pabuli
Primary publication:
Characterization and three-dimensional structures of two distinct bacterial xyloglucanases from families GH5 and GH12.
Gloster TM, Ibatullin FM, Macauley K, Eklöf JM, Roberts S, Turkenburg JP, Bjørnvad ME, Jørgensen PL, Danielsen S, Johansen KS, Borchert TV, Wilson KS, Brumer H, Davies GJ
J Biol Chem 282 19177-89 (2007)
PMID: 17376777

Function and Biology Details

Reaction catalysed: 
Xyloglucan + H(2)O = xyloglucan oligosaccharides
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-124939 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Glycoside hydrolase family 5 domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 395 amino acids
Theoretical weight: 43.97 KDa
Source organism: Paenibacillus pabuli
Expression system: Bacillus subtilis
UniProt:
  • Canonical: H9KVH3 (Residues: 1-395; Coverage: 100%)
Sequence domains: Cellulase (glycosyl hydrolase family 5)
Structure domains: Glycosidases

Ligands and Environments

Carbohydrate polymer : NEW Components: BGC, XYS, GAL
Carbohydrate polymer
No bound ligands
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: P21212
Unit cell:
a: 85.066Å b: 89.16Å c: 38.705Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.186 0.182 0.26
Expression system: Bacillus subtilis

Quick links

Citations

5 review citations

The biochemistry and structural biology of plant cell wall deconstruction.
Gilbert HJ. (2010)
4 more

10 mentions without citation

Evolution, substrate specificity and subfamily classification of glycoside hydrolase family 5 (GH5).
Aspeborg et al. (2012)
9 more