› 2jh8

X-ray diffraction
3.22Å resolution

The structure of bluetongue virus VP4 reveals a multifunctional RNA- capping production-line

Released:
DOI: 10.2210/pdb2jh8/pdb
PDB entry 2jh8 coloured by chain, front view.
PDB entry 2jh8 coloured by chain, side view.
PDB entry 2jh8 coloured by chain, top view.
Source organism: Bluetongue virus (serotype 10 / American isolate)
Primary publication:
Bluetongue virus VP4 is an RNA-capping assembly line.
Sutton G, Grimes JM, Stuart DI, Roy P
Nat Struct Mol Biol 14 449-51 (2007)
PMID: 17417654

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-179190 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Core protein VP4 Chain: A
Molecule details ›
Chain: A
Length: 644 amino acids
Theoretical weight: 75.5 KDa
Source organism: Bluetongue virus (serotype 10 / American isolate)
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q65751 (Residues: 1-644; Coverage: 100%)
Gene name: VP4
Sequence domains: Orbivirus VP4 core protein
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand M7G 1 x M7G
Ligand GUN 2 x GUN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: P6522
Unit cell:
a: 75.055Å b: 75.055Å c: 419.834Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.246 0.246 0.341
Expression system: Spodoptera frugiperda

Quick links

Citations

20 review citations

mRNA capping: biological functions and applications.
Ramanathan et al. (2016)
19 more

3 mentions without citation

Structural insights into the coupling of virion assembly and rotavirus replication.
Trask et al. (2012)
2 more