› 2jh9

X-ray diffraction
3Å resolution

The structure of bluetongue virus VP4 reveals a multifunctional RNA- capping production-line

Released:
DOI: 10.2210/pdb2jh9/pdb
PDB entry 2jh9 coloured by chain, front view.
PDB entry 2jh9 coloured by chain, side view.
PDB entry 2jh9 coloured by chain, top view.
Source organism: Bluetongue virus (serotype 10 / American isolate)
Primary publication:
Bluetongue virus VP4 is an RNA-capping assembly line.
Sutton G, Grimes JM, Stuart DI, Roy P
Nat Struct Mol Biol 14 449-51 (2007)
PMID: 17417654

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139395 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Core protein VP4 Chain: A
Molecule details ›
Chain: A
Length: 644 amino acids
Theoretical weight: 75.5 KDa
Source organism: Bluetongue virus (serotype 10 / American isolate)
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P07132 (Residues: 1-644; Coverage: 100%)
Gene name: Segment-4
Sequence domains: Orbivirus VP4 core protein
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand GTP 1 x GTP
Ligand GUN 2 x GUN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM14
Spacegroup: P6522
Unit cell:
a: 75.539Å b: 75.539Å c: 423.421Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.252 0.248 0.33
Expression system: Spodoptera frugiperda

Quick links

Citations

21 review citations

Conventional and unconventional mechanisms for capping viral mRNA.
Decroly et al. (2011)
20 more

1 mention without citation

RNA methyltransferases involved in 5' cap biosynthesis.
Byszewska et al. (2014)