› 2jhq

X-ray diffraction
1.5Å resolution

Crystal structure of Uracil DNA-glycosylase from Vibrio cholerae

Released:
DOI: 10.2210/pdb2jhq/pdb
PDB entry 2jhq coloured by chain, front view.
PDB entry 2jhq coloured by chain, side view.
PDB entry 2jhq coloured by chain, top view.
Source organism: Vibrio cholerae
Primary publication:
Structure of uracil-DNA N-glycosylase (UNG) from Vibrio cholerae: mapping temperature adaptation through structural and mutational analysis.
Raeder IL, Moe E, Willassen NP, Smalås AO, Leiros I
Acta Crystallogr Sect F Struct Biol Cryst Commun 66 130-6 (2010)
PMID: 20124707

Function and Biology Details

Reaction catalysed: 
Hydrolyzes single-stranded DNA or mismatched double-stranded DNA and polynucleotides, releasing free uracil
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-192008 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Uracil-DNA glycosylase Chain: A
Molecule details ›
Chain: A
Length: 226 amino acids
Theoretical weight: 25.48 KDa
Source organism: Vibrio cholerae
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q9KPK8 (Residues: 1-226; Coverage: 100%)
Gene names: VC_2359, ung
Sequence domains: Uracil DNA glycosylase superfamily
Structure domains: Uracil-DNA glycosylase-like domain

Ligands and Environments

1 bound ligand:
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: P212121
Unit cell:
a: 60.14Å b: 60.44Å c: 61.33Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.177 0.175 0.21
Expression system: Escherichia coli BL21

Quick links

Citations

3 mentions without citation

Staphylococcus aureus protein SAUGI acts as a uracil-DNA glycosylase inhibitor.
Wang et al. (2014)
2 more