› 2jix

X-ray diffraction
3.2Å resolution

Crystal structure of ABT-007 FAB fragment with the soluble domain of EPO receptor

Released:
DOI: 10.2210/pdb2jix/pdb
PDB entry 2jix coloured by chain, front view.
PDB entry 2jix coloured by chain, side view.
PDB entry 2jix coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
A potent erythropoietin-mimicking human antibody interacts through a novel binding site.
Liu Z, Stoll VS, Devries PJ, Jakob CG, Xie N, Simmer RL, Lacy SE, Egan DA, Harlan JE, Lesniewski RR, Reilly EB
Blood 110 2408-13 (2007)
PMID: 17620453

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-148528 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Ig-like domain-containing protein Chains: A, G, L
Molecule details ›
Chains: A, G, L
Length: 214 amino acids
Theoretical weight: 23.35 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q7Z3Y4 (Residues: 23-236; Coverage: 100%)
Sequence domains:
Structure domains: Immunoglobulins
Erythropoietin receptor Chains: B, C, E
Molecule details ›
Chains: B, C, E
Length: 225 amino acids
Theoretical weight: 24.75 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P19235 (Residues: 25-249; Coverage: 47%)
Gene name: EPOR
Sequence domains:
Structure domains: Immunoglobulins
Ig-like domain-containing protein Chains: D, F, H
Molecule details ›
Chains: D, F, H
Length: 217 amino acids
Theoretical weight: 22.95 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q6GMX6 (Residues: 20-236; Coverage: 49%)
Gene name: IGH@
Sequence domains:
Structure domains: Immunoglobulins

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 17-ID
Spacegroup: P212121
Unit cell:
a: 117.954Å b: 156.171Å c: 164.205Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.26 0.257 0.323
Expression system: Escherichia coli

Quick links

Citations

9 review citations

Next generation antibody drugs: pursuit of the 'high-hanging fruit'.
Carter et al. (2018)
8 more

7 mentions without citation

Structural and Drug Targeting Insights on Mutant p53.
Gomes et al. (2021)
6 more