› 2jjr

X-ray diffraction
2.3Å resolution

Function and Biology Details

Reaction catalysed: 
Endohydrolysis of the N-glycosidic bond at one specific adenosine on the 28S rRNA.
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140879 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ribosome-inactivating protein alpha-trichosanthin Chain: A
Molecule details ›
Chain: A
Length: 247 amino acids
Theoretical weight: 27.2 KDa
Source organism: Trichosanthes kirilowii
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P09989 (Residues: 24-270; Coverage: 93%)
Sequence domains: Ribosome inactivating protein
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand SO4 5 x SO4
Ligand PEG 5 x PEG
Ligand TAM 1 x TAM
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007
Spacegroup: P3121
Unit cell:
a: 73.64Å b: 73.64Å c: 143.443Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.186 0.184 0.214
Expression system: Escherichia coli BL21

Quick links

Citations

12 review citations

Interaction of ricin and Shiga toxins with ribosomes.
Tumer et al. (2012)
11 more

1 mention without citation

The C-terminal fragment of the ribosomal P protein complexed to trichosanthin reveals the interaction between the ribosome-inactivating protein and the ribosome.
Too et al. (2009)