› 2jlf

X-ray diffraction
3.2Å resolution

STRUCTURAL EXPLANATION FOR THE ROLE OF MN IN THE ACTIVITY OF PHI6 RNA- DEPENDENT RNA POLYMERASE

Released:
DOI: 10.2210/pdb2jlf/pdb
PDB entry 2jlf coloured by chain, front view.
PDB entry 2jlf coloured by chain, side view.
PDB entry 2jlf coloured by chain, top view.
Source organism: Pseudomonas virus phi6
Primary publication:
Structural explanation for the role of Mn2+ in the activity of phi6 RNA-dependent RNA polymerase.
Poranen MM, Salgado PS, Koivunen MR, Wright S, Bamford DH, Stuart DI, Grimes JM
Nucleic Acids Res 36 6633-44 (2008)
PMID: 18940872

Function and Biology Details

Reaction catalysed: 
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-145660 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
RNA-directed RNA polymerase Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 664 amino acids
Theoretical weight: 74.9 KDa
Source organism: Pseudomonas virus phi6
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P11124 (Residues: 2-665; Coverage: 100%)
Gene name: P2
Sequence domains: Viral RNA-dependent RNA polymerase
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand MN 2 x MN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P32
Unit cell:
a: 110.04Å b: 110.04Å c: 159.132Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.23 0.228 0.286
Expression system: Escherichia coli BL21

Quick links

Citations

5 review citations

Common and unique features of viral RNA-dependent polymerases.
te Velthuis AJ. (2014)
4 more

3 mentions without citation

Cystoviral RNA-directed RNA polymerases: Regulation of RNA synthesis on multiple time and length scales.
Alphonse et al. (2017)
2 more