› 2jo6

Solution NMR

NMR structure of the E.coli protein NirD, Northeast Structural Genomics target ET100

Released:
DOI: 10.2210/pdb2jo6/pdb
PDB entry 2jo6 coloured by chain, ensemble of 20 models, front view.
PDB entry 2jo6 coloured by chain, ensemble of 20 models, side view.
PDB entry 2jo6 coloured by chain, ensemble of 20 models, top view.
Source organism: Escherichia coli
Entry authors: Ramelot TA, Cort JR, Yee AA, Guido V, Lukin JA, Arrowsmith CH, Kennedy MA, Northeast Structural Genomics Consortium (NESG)

Function and Biology Details

Reaction catalysed: 
Ammonia + 3 NAD(+) + 2 H(2)O = nitrite + 3 NADH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-141888 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nitrite reductase (NADH) small subunit Chain: A
Molecule details ›
Chain: A
Length: 113 amino acids
Theoretical weight: 12.77 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A9I8 (Residues: 1-108; Coverage: 100%)
Gene names: JW3329, b3366, nirD
Sequence domains: Rieske-like [2Fe-2S] domain
Structure domains: Rieske [2Fe-2S] iron-sulphur domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this NMR entry.
Chemical shift assignment: 93%
Refinement method: simulated annealing
Expression system: Escherichia coli

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