Function and Biology Details
Reaction catalysed:
(1a) S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [HECT-type E3 ubiquitin transferase]-L-cysteine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + S-ubiquitinyl-[HECT-type E3 ubiquitin transferase]-L-cysteine
Biochemical function:
- not assigned
Biological process:
- not assigned
Cellular component:
- not assigned
Sequence domains:
Structure analysis Details
Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146535 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
E3 ubiquitin-protein ligase Itchy
Molecule details ›
Chain: A
Length: 37 amino acids
Theoretical weight: 4.28 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21
UniProt:
Sequence domains: WW domain
Length: 37 amino acids
Theoretical weight: 4.28 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21
UniProt:
- Canonical:
Q8C863 (Residues: 399-432; Coverage: 4%)
Sequence domains: WW domain
Latent membrane protein 2
Molecule details ›
Chain: B
Length: 9 amino acids
Theoretical weight: 1.07 KDa
Source organism: Human herpesvirus 4 strain B95-8
Expression system: Not provided
UniProt:
Length: 9 amino acids
Theoretical weight: 1.07 KDa
Source organism: Human herpesvirus 4 strain B95-8
Expression system: Not provided
UniProt:
- Canonical: P13285 (Residues: 54-62; Coverage: 2%)
Ligands and Environments
No bound ligands
No modified residues
Experiments and Validation Details
Refinement method:
simulated annealing
Expression systems:
- Escherichia coli BL21
- Not provided
