› 2jpa

Solution NMR

Structure of the Wilms Tumor Suppressor Protein Zinc Finger Domain Bound to DNA

Released:
DOI: 10.2210/pdb2jpa/pdb
PDB entry 2jpa coloured by chain, ensemble of 20 models, front view.
PDB entry 2jpa coloured by chain, ensemble of 20 models, side view.
PDB entry 2jpa coloured by chain, ensemble of 20 models, top view.
Source organisms:
Primary publication:
Structure of the Wilms tumor suppressor protein zinc finger domain bound to DNA.
Stoll R, Lee BM, Debler EW, Laity JH, Wilson IA, Dyson HJ, Wright PE
J Mol Biol 372 1227-45 (2007)
PMID: 17716689

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-115431 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Wilms tumor protein Chain: A
Molecule details ›
Chain: A
Length: 119 amino acids
Theoretical weight: 14.49 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P19544 (Residues: 318-438; Coverage: 26%)
Gene name: WT1
Sequence domains: Zinc finger, C2H2 type
Structure domains: Classic Zinc Finger
DNA (5'-D(P*DCP*DGP*DCP*DGP*DGP*DGP*DGP*DGP*DCP*DGP*DTP*DCP*DTP*DG)-3') Chain: B
Molecule details ›
Chain: B
Length: 14 nucleotides
Theoretical weight: 4.35 KDa
Source organism: synthetic construct
Expression system: Not provided
DNA (5'-D(P*DCP*DAP*DGP*DAP*DCP*DGP*DCP*DCP*DCP*DCP*DCP*DGP*DCP*DG)-3') Chain: C
Molecule details ›
Chain: C
Length: 14 nucleotides
Theoretical weight: 4.21 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand ZN 4 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 11%
Refinement method: molecular dynamics
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

8 review citations

Wilms' tumours: about tumour suppressor genes, an oncogene and a chameleon gene.
Huff V. (2011)
7 more

3 mentions without citation

An expanded binding model for Cys2His2 zinc finger protein-DNA interfaces.
Persikov et al. (2011)
2 more