› 2jvo

Solution NMR

Segmental isotope labeling of Npl3

Released:
DOI: 10.2210/pdb2jvo/pdb
PDB entry 2jvo coloured by chain, ensemble of 20 models, front view.
PDB entry 2jvo coloured by chain, ensemble of 20 models, side view.
PDB entry 2jvo coloured by chain, ensemble of 20 models, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Improved segmental isotope labeling methods for the NMR study of multidomain or large proteins: application to the RRMs of Npl3p and hnRNP L.
Skrisovska L, Allain FH
J Mol Biol 375 151-64 (2008)
PMID: 17936301

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-169592 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Serine/arginine (SR)-type shuttling mRNA binding protein NPL3 Chain: A
Molecule details ›
Chain: A
Length: 108 amino acids
Theoretical weight: 12.29 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: Q01560 (Residues: 116-201; Coverage: 21%)
Gene names: D9461.19, MTR13, MTS1, NAB1, NOP3, NPL3, YDR432W
Sequence domains: RNA recognition motif
Structure domains: Alpha-Beta Plaits

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 82%
Refinement method: simulated annealing
Expression system: Escherichia coli

Quick links

Citations

8 review citations

Chemoenzymatic Semisynthesis of Proteins.
Thompson et al. (2020)
7 more

1 mention without citation

Malleable ribonucleoprotein machine: protein intrinsic disorder in the Saccharomyces cerevisiae spliceosome.
Coelho Ribeiro Mde et al. (2013)