› 2jx8

Solution NMR

Solution structure of hPCIF1 WW domain

Released:
DOI: 10.2210/pdb2jx8/pdb
PDB entry 2jx8 coloured by chain, ensemble of 20 models, front view.
PDB entry 2jx8 coloured by chain, ensemble of 20 models, side view.
PDB entry 2jx8 coloured by chain, ensemble of 20 models, top view.
Source organism: Homo sapiens
Entry authors: Kouno T, Iwamoto Y, Hirose Y, Aizawa T, Demura M, Kawano K, Ohkuma Y, Mizuguchi M

Function and Biology Details

Reaction catalysed: 
S-adenosyl-L-methionine + a 5-(N(7)-methyl 5-triphosphoguanosine)-2'-O-methyladenosine-[mRNA] = S-adenosyl-L-homocysteine + a 5-(N(7)-methyl 5-triphosphoguanosine)-N(6),2'-O-dimethyladenosine-[mRNA]
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-190622 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
mRNA (2'-O-methyladenosine-N(6)-)-methyltransferase Chain: A
Molecule details ›
Chain: A
Length: 52 amino acids
Theoretical weight: 6.15 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9H4Z3 (Residues: 40-86; Coverage: 7%)
Gene names: C20orf67, CAPAM, PCIF1, PPP1R121
Sequence domains: WW domain
Structure domains: Ubiquitin Ligase Nedd4; Chain: W;

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 83%
Refinement method: simulated annealing
Expression system: Escherichia coli

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