› 2jxl

Solution NMR

Solution structure of cardiac N-domain troponin C mutant F77W-V82A

Released:
DOI: 10.2210/pdb2jxl/pdb
PDB entry 2jxl coloured by chain, ensemble of 30 models, front view.
PDB entry 2jxl coloured by chain, ensemble of 30 models, side view.
PDB entry 2jxl coloured by chain, ensemble of 30 models, top view.
Source organism: Homo sapiens
Primary publication:
Tryptophan mutants of cardiac troponin C: 3D structure, troponin I affinity, and in situ activity.
Julien O, Sun YB, Wang X, Lindhout DA, Thiessen A, Irving M, Sykes BD
Biochemistry 47 597-606 (2008)
PMID: 18092822

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-159088 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Troponin C, slow skeletal and cardiac muscles Chain: A
Molecule details ›
Chain: A
Length: 89 amino acids
Theoretical weight: 10.08 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P63316 (Residues: 1-89; Coverage: 55%)
Gene names: TNNC, TNNC1
Sequence domains: EF-hand domain pair
Structure domains: EF-hand

Ligands and Environments

1 bound ligand:
Ligand CA 1 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: torsion angle dynamics
Expression system: Escherichia coli

Quick links

Citations

1 citations in other articles

Tryptophan side chain conformers monitored by NMR and time-resolved fluorescence spectroscopies.
Julien et al. (2012)

1 mention without citation

The effect of the cosolvent trifluoroethanol on a tryptophan side chain orientation in the hydrophobic core of troponin C.
Julien et al. (2009)