› 2jy9

Solution NMR

NMR structure of putative tRNA hydrolase domain from Salmonella typhimurium. NorthEast Structural Genomics Consortium target StR220

Released:
DOI: 10.2210/pdb2jy9/pdb
PDB entry 2jy9 coloured by chain, ensemble of 20 models, front view.
PDB entry 2jy9 coloured by chain, ensemble of 20 models, side view.
PDB entry 2jy9 coloured by chain, ensemble of 20 models, top view.
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
Entry authors: Singarapu KK, Wu Y, Sukumaran D, Eletsky A, Zeri A, Wang D, Janjua H, Owens L, Xiao R, Liu J, Baran MC, Swapna GVT, Acton TB, Rost B, Montelione GT, Szyperski T, Northeast Structural Genomics Consortium (NESG)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-187388 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Prokaryotic-type class I peptide chain release factors domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 148 amino acids
Theoretical weight: 16.7 KDa
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q8ZRN3 (Residues: 1-140; Coverage: 100%)
Gene names: STM0240, yaeJ
Sequence domains: RF-1 domain
Structure domains: Double Stranded RNA Binding Domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 87%
Refinement method: simulated annealing
Expression system: Escherichia coli BL21

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