Function and Biology Details
Reaction catalysed:
[Protein]-N(pi)-phospho-L-histidine + D-mannose(Side 1) = [protein]-L-histidine + D-mannose 6-phosphate(Side 2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
- Phosphotransferase system, mannose-type IIA component superfamily
- Phosphotransferase system, mannose-type IIA component
- Phosphotransferase system, sorbose subfamily IIB component
- Phosphotransferase system, sorbose subfamily IIB component superfamily
- PTS system mannose/sorbose specific IIA subunit
- Phosphotransferase system, mannose family IIA component
- Phosphotransferase system, sorbose subfamily IIB component, subgroup
Structure analysis Details
Assembly composition:
hetero trimer (preferred)
Assembly name:
PTS system mannose-specific EIIAB component (preferred)
PDBe Complex ID:
PDB-CPX-159666 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Ligands and Environments
No bound ligands
No modified residues
Experiments and Validation Details
Refinement method:
conjoined rigid body/torsion angle simulated annealing
Expression system: Escherichia coli