› 2k0d

Solution NMR

NMR structure of a mutant colicin e7 immunity protein im7 with an extended helix III

Released:
DOI: 10.2210/pdb2k0d/pdb
PDB entry 2k0d coloured by chain, ensemble of 30 models, front view.
PDB entry 2k0d coloured by chain, ensemble of 30 models, side view.
PDB entry 2k0d coloured by chain, ensemble of 30 models, top view.
Source organism: Escherichia coli
Primary publication:
Amino acid insertion reveals a necessary three-helical intermediate in the folding pathway of the colicin E7 immunity protein Im7.
Knowling SE, Figueiredo AM, Whittaker SB, Moore GR, Radford SE
J Mol Biol 392 1074-86 (2009)
PMID: 19651139

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-169933 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Colicin-E7 immunity protein Chain: X
Molecule details ›
Chain: X
Length: 101 amino acids
Theoretical weight: 11.09 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: Q03708 (Residues: 2-58, 59-87; Coverage: 99%)
Gene names: ceiE7, imm
Sequence domains: Colicin immunity protein / pyocin immunity protein
Structure domains: Colicin E immunity protein

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: torsion angle dynamics, simulated annealing
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Nuclease colicins and their immunity proteins.
Papadakos et al. (2012)
6 other articles

2 mentions without citation

Amino acid insertion reveals a necessary three-helical intermediate in the folding pathway of the colicin E7 immunity protein Im7.
Knowling et al. (2009)
1 more