› 2k1w

Solution NMR

NMR solution structure of M-crystallin in calcium loaded form(holo).

Released:
DOI: 10.2210/pdb2k1w/pdb
PDB entry 2k1w coloured by chain, ensemble of 20 models, front view.
PDB entry 2k1w coloured by chain, ensemble of 20 models, side view.
PDB entry 2k1w coloured by chain, ensemble of 20 models, top view.
Source organism: Methanosarcina acetivorans
Primary publication:
Solution structure and calcium-binding properties of M-crystallin, a primordial betagamma-crystallin from archaea.
Barnwal RP, Jobby MK, Devi KM, Sharma Y, Chary KV
J Mol Biol 386 675-89 (2009)
PMID: 19138688

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-186288 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Beta/gamma crystallin 'Greek key' domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 85 amino acids
Theoretical weight: 9.38 KDa
Source organism: Methanosarcina acetivorans
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8TMX3 (Residues: 37-120; Coverage: 70%)
Gene name: MA_2526
Sequence domains: Beta/Gamma crystallin
Structure domains: Crystallins

Ligands and Environments

1 bound ligand:
Ligand CA 2 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: torsion angle dynamics, simulated annealing
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Calcium binding proteins and calcium signaling in prokaryotes.
Domínguez et al. (2015)
3 more

5 mentions without citation

Explosive expansion of betagamma-crystallin genes in the ancestral vertebrate.
Kappé et al. (2010)
4 more