› 2k66

Solution NMR

NMR solution structure of the d3'-stem closed by a GAAA tetraloop of the group II intron Sc.ai5(gamma)

Released:
DOI: 10.2210/pdb2k66/pdb
PDB entry 2k66 coloured by chain, ensemble of 20 models, front view.
PDB entry 2k66 coloured by chain, ensemble of 20 models, side view.
PDB entry 2k66 coloured by chain, ensemble of 20 models, top view.
Source organism: Synthetic construct
Primary publication:
NMR structure of the 5' splice site in the group IIB intron Sc.ai5γ--conformational requirements for exon-intron recognition.
Kruschel D, Skilandat M, Sigel RK
RNA 20 295-307 (2014)
PMID: 24448450

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-196411 (preferred)
Entry contents:
1 distinct RNA molecule
Macromolecule:
5'-R(*GP*GP*AP*GP*UP*AP*UP*GP*UP*GP*AP*AP*AP*GP*CP*AP*UP*AP*CP*UP*CP*C)-3' Chain: A
Molecule details ›
Chain: A
Length: 22 nucleotides
Theoretical weight: 7.08 KDa
Source organism: Synthetic construct
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: torsion angle dynamics, simulated annealing
Expression system: Not provided

Quick links

Citations

2 review citations

NMR Studies of the Structure and Function of the HIV-1 5'-Leader.
Keane et al. (2016)
1 more

2 mentions without citation

Major groove width variations in RNA structures determined by NMR and impact of 13C residual chemical shift anisotropy and 1H-13C residual dipolar coupling on refinement.
Tolbert et al. (2010)
1 more