› 2ka7

Solution NMR

NMR solution structure of TM0212 at 40 C

Released:
DOI: 10.2210/pdb2ka7/pdb
PDB entry 2ka7 coloured by chain, ensemble of 20 models, front view.
PDB entry 2ka7 coloured by chain, ensemble of 20 models, side view.
PDB entry 2ka7 coloured by chain, ensemble of 20 models, top view.
Source organism: Thermotoga maritima
Primary publication:
The J-UNIO protocol for automated protein structure determination by NMR in solution.
Serrano P, Pedrini B, Mohanty B, Geralt M, Herrmann T, Wüthrich K
J Biomol NMR 53 341-54 (2012)
PMID: 22752932

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-194462 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glycine cleavage system H protein Chain: A
Molecule details ›
Chain: A
Length: 124 amino acids
Theoretical weight: 13.93 KDa
Source organism: Thermotoga maritima
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9WY55 (Residues: 1-124; Coverage: 100%)
Gene names: TM_0212, gcvH
Sequence domains: Glycine cleavage H-protein
Structure domains: OB fold (Dihydrolipoamide Acetyltransferase, E2P)

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 83%
Refinement method: molecular dynamics
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Modern Technologies of Solution Nuclear Magnetic Resonance Spectroscopy for Three-dimensional Structure Determination of Proteins Open Avenues for Life Scientists.
Sugiki et al. (2017)
1 more

1 mention without citation

The J-UNIO protocol for automated protein structure determination by NMR in solution.
Serrano et al. (2012)