› 2kb6

Solution NMR

Solution structure of onconase C87A/C104A

Released:
DOI: 10.2210/pdb2kb6/pdb
PDB entry 2kb6 coloured by chain, ensemble of 10 models, front view.
PDB entry 2kb6 coloured by chain, ensemble of 10 models, side view.
PDB entry 2kb6 coloured by chain, ensemble of 10 models, top view.
Source organism: Lithobates pipiens
Primary publication:
Impact of the C-terminal disulfide bond on the folding and stability of onconase.
Schulenburg C, Weininger U, Neumann P, Meiselbach H, Stubbs MT, Sticht H, Balbach J, Ulbrich-Hofmann R, Arnold U
Chembiochem 11 978-86 (2010)
PMID: 20349493

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149514 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Protein P-30 Chain: A
Molecule details ›
Chain: A
Length: 104 amino acids
Theoretical weight: 11.78 KDa
Source organism: Lithobates pipiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P22069 (Residues: 1-104; Coverage: 100%)
Sequence domains: Pancreatic ribonuclease
Structure domains: Ribonuclease A-like domain

Ligands and Environments

No bound ligands
1 modified residue:
Ligand PCA 1 x PCA

Experiments and Validation Details

Entry percentile scores
Refinement method: simulated annealing
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

Stability and folding of amphibian ribonuclease A superfamily members in comparison with mammalian homologues.
Arnold U. (2014)
1 more