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Solution NMR

SOLUTION STRUCTURE OF 30S RIBOSOMAL PROTEIN S8E; FROM Methanothermobacter thermautotrophicus, NORTHEASTSTRUCTURAL GENOMICS CONSORTIUM (NESG) TARGET Tr71d

Released:
DOI: 10.2210/pdb2kcp/pdb
PDB entry 2kcp coloured by chain, ensemble of 20 models, front view.
PDB entry 2kcp coloured by chain, ensemble of 20 models, side view.
PDB entry 2kcp coloured by chain, ensemble of 20 models, top view.
Source organism: Methanothermobacter thermautotrophicus str. Delta H
Entry authors: Liu G, Rossi P, Wang D, Nwosu C, Owens L, Xiao R, Liu J, Baran MC, Swapna G, Acton TB, Rost B, Montelione GT, Northeast Structural Genomics Consortium (NESG)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-127831 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Small ribosomal subunit protein eS8 Chain: A
Molecule details ›
Chain: A
Length: 98 amino acids
Theoretical weight: 11.04 KDa
Source organism: Methanothermobacter thermautotrophicus str. Delta H
Expression system: Escherichia coli
UniProt:
  • Canonical: O26309 (Residues: 38-127; Coverage: 71%)
Gene names: MTH_207, rps8e
Sequence domains: Ribosomal protein S8e
Structure domains: Thrombin, subunit H

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 84%
Refinement method: distance geometry, simulated annealing, molecular dynamics, torsion angle dynamics
Expression system: Escherichia coli

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