› 2khh

Solution NMR

Structural requirements for the UBA domain of the mRNA export factor Mex67 to bind its specific targets, the transcription elongation Tho complex component Hpr1 and nucleoporin FxFG repeats

Released:
DOI: 10.2210/pdb2khh/pdb
PDB entry 2khh coloured by chain, ensemble of 20 models, front view.
PDB entry 2khh coloured by chain, ensemble of 20 models, side view.
PDB entry 2khh coloured by chain, ensemble of 20 models, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Structural requirements for the ubiquitin-associated domain of the mRNA export factor Mex67 to bind its specific targets, the transcription elongation THO complex component Hpr1 and nucleoporin FXFG repeats.
Hobeika M, Brockmann C, Gruessing F, Neuhaus D, Divita G, Stewart M, Dargemont C
J Biol Chem 284 17575-83 (2009)
PMID: 19401465

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-162818 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
mRNA export factor MEX67 Chain: A
Molecule details ›
Chain: A
Length: 59 amino acids
Theoretical weight: 6.82 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: Q99257 (Residues: 543-599; Coverage: 10%)
Gene names: MEX67, P2520, YPL169C
Sequence domains: TAP C-terminal domain
Structure domains: DNA helicase RuvA subunit, C-terminal domain
FxFG Chain: B
Molecule details ›
Chain: B
Length: 9 amino acids
Theoretical weight: 932 Da
Source organism: Saccharomyces cerevisiae
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: simulated annealing
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

3 review citations

Nuclear export of mRNA.
Stewart M. (2010)
2 more

2 mentions without citation

A model of a MAPK•substrate complex in an active conformation: a computational and experimental approach.
Lee et al. (2011)
1 more