› 2khs

Solution NMR

Solution structure of SNase121:SNase(111-143) complex

Released:
DOI: 10.2210/pdb2khs/pdb
PDB entry 2khs coloured by chain, ensemble of 20 models, front view.
PDB entry 2khs coloured by chain, ensemble of 20 models, side view.
PDB entry 2khs coloured by chain, ensemble of 20 models, top view.
Source organism: Staphylococcus aureus
Primary publication:
The native-like interactions between SNase121 and SNase(111-143) fragments induce the recovery of their native-like structures and the ability to degrade DNA.
Geng Y, Feng Y, Xie T, Shan L, Wang J
Biochemistry 48 8692-703 (2009)
PMID: 19658434

Function and Biology Details

Reaction catalysed: 
Endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotide end-products
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132791 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Nuclease A Chain: A
Molecule details ›
Chain: A
Length: 121 amino acids
Theoretical weight: 13.61 KDa
Source organism: Staphylococcus aureus
Expression system: Escherichia coli
UniProt:
  • Canonical: P00644 (Residues: 83-203; Coverage: 59%)
Gene name: nuc
Sequence domains: Staphylococcal nuclease homologue
Structure domains: OB fold (Dihydrolipoamide Acetyltransferase, E2P)
Thermonuclease Chain: B
Molecule details ›
Chain: B
Length: 35 amino acids
Theoretical weight: 4.08 KDa
Source organism: Staphylococcus aureus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q1WCB7 (Residues: 190-222; Coverage: 15%)
Gene name: nuc

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 62%
Refinement method: molecular dynamics, simulated annealing
Expression system: Escherichia coli

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