› 2klh

Solution NMR

NMR Structure of RCL in complex with GMP

Released:
DOI: 10.2210/pdb2klh/pdb
PDB entry 2klh coloured by chain, ensemble of 10 models, front view.
PDB entry 2klh coloured by chain, ensemble of 10 models, side view.
PDB entry 2klh coloured by chain, ensemble of 10 models, top view.
Source organism: Rattus norvegicus
Primary publication:
Structural characterization of the mammalian deoxynucleotide N-hydrolase Rcl and its stabilizing interactions with two inhibitors.
Yang Y, Padilla A, Zhang C, Labesse G, Kaminski PA
J Mol Biol 394 435-47 (2009)
PMID: 19822152

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-128714 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
5-hydroxymethyl-dUMP N-hydrolase Chains: A, B
Molecule details ›
Chains: A, B
Length: 152 amino acids
Theoretical weight: 17.13 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: O35820 (Residues: 11-151; Coverage: 87%)
Gene names: Dnph1, Rcl
Sequence domains: Nucleoside 2-deoxyribosyltransferase
Structure domains: Rossmann fold

Ligands and Environments

1 bound ligand:
Ligand 5GP 2 x 5GP
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: torsion angle dynamics
Expression system: Escherichia coli

Quick links

Citations

2 review citations

8-Azapurines as isosteric purine fluorescent probes for nucleic acid and enzymatic research.
Wierzchowski et al. (2014)
1 more

2 mentions without citation

Reversal of the substrate specificity of CMP N-glycosidase to dCMP.
Sikowitz et al. (2013)
1 more