› 2ko0

Solution NMR

Solution structure of the THAP zinc finger of THAP1 in complex with its DNA target

Released:
DOI: 10.2210/pdb2ko0/pdb
PDB entry 2ko0 coloured by chain, ensemble of 15 models, front view.
PDB entry 2ko0 coloured by chain, ensemble of 15 models, side view.
PDB entry 2ko0 coloured by chain, ensemble of 15 models, top view.
Source organism: Homo sapiens
Primary publication:
Structural determinants of specific DNA-recognition by the THAP zinc finger.
Campagne S, Saurel O, Gervais V, Milon A
Nucleic Acids Res 38 3466-76 (2010)
PMID: 20144952

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-115494 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
THAP domain-containing protein 1 Chain: A
Molecule details ›
Chain: A
Length: 87 amino acids
Theoretical weight: 10.19 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9NVV9 (Residues: 1-82; Coverage: 39%)
Gene name: THAP1
Sequence domains: THAP domain
RRM1 Chain: B
Molecule details ›
Chain: B
Length: 16 nucleotides
Theoretical weight: 4.99 KDa
Source organism: Homo sapiens
Expression system: Not provided
ZINC ION Chain: C
Molecule details ›
Chain: C
Length: 16 nucleotides
Theoretical weight: 4.81 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand ZN 1 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: Rigid body docking, Semi flexible simulated annealing, Water refinement
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

9 review citations

Molecular pathways in dystonia.
Bragg et al. (2011)
8 more

4 mentions without citation

Nuclear magnetic resonance analysis of protein-DNA interactions.
Campagne et al. (2011)
3 more