› 2ksq

Solution NMR

The myristoylated yeast ARF1 in a GTP and bicelle bound conformation

Released:
DOI: 10.2210/pdb2ksq/pdb
PDB entry 2ksq coloured by chain, ensemble of 20 models, front view.
PDB entry 2ksq coloured by chain, ensemble of 20 models, side view.
PDB entry 2ksq coloured by chain, ensemble of 20 models, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Dynamic structure of membrane-anchored Arf*GTP.
Liu Y, Kahn RA, Prestegard JH
Nat. Struct. Mol. Biol. 17 876-81 (2010)
PMID: 20601958

Function and Biology Details

Reaction catalysed: 
GTP + H(2)O = GDP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-145648 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
ADP-ribosylation factor 1 Chain: A
Molecule details ›
Chain: A
Length: 181 amino acids
Theoretical weight: 20.52 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P11076 (Residues: 2-181; Coverage: 99%)
Gene names: ARF1, D1244, YDL192W
Sequence domains: ADP-ribosylation factor family
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

2 bound ligands:
Ligand MTN 5 x MTN
Ligand GTP 1 x GTP
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: simulated annealing
Expression system: Escherichia coli

Quick links

Citations

17 review citations

Structure-function relationships of the G domain, a canonical switch motif.
Wittinghofer et al. (2011)
16 more

12 mentions without citation

The magic of bicelles lights up membrane protein structure.
Dürr et al. (2012)
11 more