› 2kv7

Solution NMR

NMR solution structure of a soluble PrgI mutant from Salmonella Typhimurium

Released:
DOI: 10.2210/pdb2kv7/pdb
PDB entry 2kv7 coloured by chain, ensemble of 25 models, front view.
PDB entry 2kv7 coloured by chain, ensemble of 25 models, side view.
PDB entry 2kv7 coloured by chain, ensemble of 25 models, top view.
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium str. SL1344
Primary publication:
Protein refolding is required for assembly of the type three secretion needle.
Poyraz O, Schmidt H, Seidel K, Delissen F, Ader C, Tenenboim H, Goosmann C, Laube B, Thünemann AF, Zychlinsky A, Baldus M, Lange A, Griesinger C, Kolbe M
Nat Struct Mol Biol 17 788-92 (2010)
PMID: 20543831

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-154437 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
SPI-1 type 3 secretion system needle filament protein Chain: A
Molecule details ›
Chain: A
Length: 83 amino acids
Theoretical weight: 9.09 KDa
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium str. SL1344
Expression system: Escherichia coli
UniProt:
  • Canonical: P41784 (Residues: 1-80; Coverage: 100%)
Gene names: STM2873, prgI, sctF1
Sequence domains: Type III secretion needle MxiH, YscF, SsaG, EprI, PscF, EscF

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: simulated annealing
Expression system: Escherichia coli

Quick links

Citations

16 review citations

Secretion systems in Gram-negative bacteria: structural and mechanistic insights.
Costa et al. (2015)
15 more

1 mention without citation

Protein Structure Idealization: How accurately is it possible to model protein structures with dihedral angles?
Cui et al. (2013)