› 2kwg

Solution NMR

Solution structure of a fully modified 2'-F/2'-OMe siRNA construct

Released:
DOI: 10.2210/pdb2kwg/pdb
PDB entry 2kwg coloured by chain, ensemble of 10 models, front view.
PDB entry 2kwg coloured by chain, ensemble of 10 models, side view.
PDB entry 2kwg coloured by chain, ensemble of 10 models, top view.
Source organism: Synthetic construct
Primary publication:
Solution-state structure of a fully alternately 2'-F/2'-OMe modified 42-nt dimeric siRNA construct.
Podbevsek P, Allerson CR, Bhat B, Plavec J
Nucleic Acids Res 38 7298-307 (2010)
PMID: 20624819

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-196436 (preferred)
Entry contents:
2 distinct RNA molecules
Macromolecules (2 distinct):
5'-R(*(GF2)P*(OMG)P*(GF2)P*(OMU)P*(AF2)P*(A2M)P*(AF2)P*(OMU)P*(AF2)P*(OMC)P*(AF2)P*(OMU)P*(UFT)P*(OMC)P*(UFT)P*(OMU)P*(CFZ)P*(A2M)P*(UFT)P*(OMU)P*(UFT))-3' Chain: A
Molecule details ›
Chain: A
Length: 21 nucleotides
Theoretical weight: 6.8 KDa
Source organism: Synthetic construct
Expression system: Not provided
5'-R(P*(A2M)P*(UFT)P*(OMG)P*(AF2)P*(A2M)P*(GF2)P*(A2M)P*(AF2)P*(OMU)P*(GF2)P*(OMU)P*(AF2)P*(OMU)P*(UFT)P*(OMU)P*(AF2)P*(OMC)P*(CFZ)P*(OMC)P*(UFT)P*(OMU))-3' Chain: B
Molecule details ›
Chain: B
Length: 21 nucleotides
Theoretical weight: 6.83 KDa
Source organism: Synthetic construct
Expression system: Not provided

Ligands and Environments

No bound ligands
8 modified residues:
Ligand GF2 4 x GF2
Ligand OMG 2 x OMG
Ligand OMU 10 x OMU
Ligand AF2 8 x AF2
Ligand A2M 5 x A2M
Ligand OMC 4 x OMC
Ligand UFT 7 x UFT
Ligand CFZ 2 x CFZ

Experiments and Validation Details

Entry percentile scores
Refinement method: simulated annealing
Expression system: Not provided

Quick links

Citations

2 citation in other articles

Structural studies of the formation of lipoplexes between siRNA and selected bis-imidazolium gemini surfactants.
Andrzejewska et al. (2016)
1 more

2 mentions without citation

Improving NMR Structures of RNA.
Bermejo et al. (2016)
1 more