› 2kxg

Solution NMR

The solution structure of the squash aspartic acid proteinase inhibitor (SQAPI)

Released:
DOI: 10.2210/pdb2kxg/pdb
PDB entry 2kxg coloured by chain, ensemble of 10 models, front view.
PDB entry 2kxg coloured by chain, ensemble of 10 models, side view.
PDB entry 2kxg coloured by chain, ensemble of 10 models, top view.
Source organism: Cucurbita maxima
Primary publication:
Solution structure of the squash aspartic acid proteinase inhibitor (SQAPI) and mutational analysis of pepsin inhibition.
Headey SJ, MacAskill UK, Wright MA, Claridge JK, Edwards PJB, Farley PC, Christeller JT, Laing WA, Pascal SM
J Biol Chem 285 27019-27025 (2010)
PMID: 20538608

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-179518 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cystatin domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 95 amino acids
Theoretical weight: 10.42 KDa
Source organism: Cucurbita maxima
Expression system: Escherichia coli
UniProt:
  • Canonical: Q6DLC9 (Residues: 8-102; Coverage: 93%)
Sequence domains: Aspartic acid proteinase inhibitor
Structure domains: Nuclear Transport Factor 2; Chain: A,

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 84%
Refinement method: torsion angle dynamics, simulated annealing, simulated annealing
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Peptide-based protease inhibitors from plants.
Hellinger et al. (2019)
1 more

1 mention without citation

Solution structure of the squash aspartic acid proteinase inhibitor (SQAPI) and mutational analysis of pepsin inhibition.
Headey et al. (2010)