› 2kyr

Solution NMR

Solution structure of Enzyme IIB subunit of PTS system from Escherichia coli K12. Northeast Structural Genomics Consortium target ER315/Ontario Center for Structural Proteomics target ec0544

Released:
DOI: 10.2210/pdb2kyr/pdb
PDB entry 2kyr coloured by chain, ensemble of 20 models, front view.
PDB entry 2kyr coloured by chain, ensemble of 20 models, side view.
PDB entry 2kyr coloured by chain, ensemble of 20 models, top view.
Source organism: Escherichia coli K-12
Entry authors: Wu B, Yee A, Gutmanas A, Semest A, Montelione GT, Arrowsmith CH, Northeast Structural Genomics Consortium (NESG), Ontario Centre for Structural Proteomics (OCSP)

Function and Biology Details

Reaction catalysed: 
[Protein]-N(pi)-phospho-L-histidine + D-fructose(Side 1) = [protein]-L-histidine + D-fructose 1-phosphate(Side 2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-159670 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
PTS system fructose-like EIIB component 1 Chain: A
Molecule details ›
Chain: A
Length: 111 amino acids
Theoretical weight: 12.07 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P69808 (Residues: 1-108; Coverage: 100%)
Gene names: JW5389, b2387, fryB, ypdH
Sequence domains: PTS system, Lactose/Cellobiose specific IIB subunit
Structure domains: Rossmann fold

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 93%
Refinement method: simulated annealing
Expression system: Escherichia coli

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