2kz7

Solution NMR

Solution structure of the CARMIL CAH3a/b domain bound to capping protein (CP)

Released:
Source organisms:
Entry authors: Zwolak A, Tjandra N

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-146480 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
F-actin-capping protein subunit alpha-1 Chain: A
Molecule details ›
Chain: A
Length: 286 amino acids
Theoretical weight: 33 KDa
Source organism: Gallus gallus
Expression system: Escherichia coli
UniProt:
  • Canonical: P13127 (Residues: 1-286; Coverage: 100%)
Gene name: CAPZA1
Sequence domains: F-actin capping protein alpha subunit
Structure domains:
F-actin-capping protein subunit beta isoforms 1 and 2 Chain: B
Molecule details ›
Chain: B
Length: 277 amino acids
Theoretical weight: 31.4 KDa
Source organism: Gallus gallus
Expression system: Escherichia coli
UniProt:
  • Canonical: P14315 (Residues: 1-277; Coverage: 100%)
Gene name: CAPZB
Sequence domains: F-actin capping protein, beta subunit
Structure domains:
F-actin-uncapping protein LRRC16A Chain: C
Molecule details ›
Chain: C
Length: 85 amino acids
Theoretical weight: 9.45 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q6EDY6 (Residues: 965-1039; Coverage: 6%)
Gene names: Carmil, Carmil1, Lrrc16, Lrrc16a

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: molecular dynamics, simulated annealing, torsion angle dynamics
Expression system: Escherichia coli