› 2l2b

Solution NMR

Structure of StnII-Y111N, a mutant of the sea anemone actinoporin Sticholysin II

Released:
DOI: 10.2210/pdb2l2b/pdb
PDB entry 2l2b coloured by chain, ensemble of 20 models, front view.
PDB entry 2l2b coloured by chain, ensemble of 20 models, side view.
PDB entry 2l2b coloured by chain, ensemble of 20 models, top view.
Source organism: Stichodactyla helianthus
Primary publication:
Intrinsic local disorder and a network of charge-charge interactions are key to actinoporin membrane disruption and cytotoxicity.
Pardo-Cea MA, Castrillo I, Alegre-Cebollada J, Martínez-del-Pozo Á, Gavilanes JG, Bruix M
FEBS J 278 2080-9 (2011)
PMID: 21481191

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139789 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DELTA-stichotoxin-She4b Chain: A
Molecule details ›
Chain: A
Length: 175 amino acids
Theoretical weight: 19.25 KDa
Source organism: Stichodactyla helianthus
Expression system: Escherichia coli
UniProt:
  • Canonical: P07845 (Residues: 1-175; Coverage: 100%)
Sequence domains: Sea anemone cytotoxic protein
Structure domains: Cytolysin/lectin

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this NMR entry.
Refinement method: molecular dynamics
Expression system: Escherichia coli

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Citations

12 citation in other articles

Membrane damage by an α-helical pore-forming protein, Equinatoxin II, proceeds through a succession of ordered steps.
Rojko et al. (2013)
11 more