› 2l2d

Solution NMR

Solution NMR Structure of human UBA-like domain of OTUD7A_11_83, NESG target HT6304A/OCSP target OTUD7A_11_83/SGC-Toronto

Released:
DOI: 10.2210/pdb2l2d/pdb
PDB entry 2l2d coloured by chain, ensemble of 20 models, front view.
PDB entry 2l2d coloured by chain, ensemble of 20 models, side view.
PDB entry 2l2d coloured by chain, ensemble of 20 models, top view.
Source organism: Homo sapiens
Entry authors: Wu B, Yee A, Lemak A, Gutmanas A, Houliston S, Semesi A, Dhe-Paganon S, Montelione GT, Arrowsmith CH, Northeast Structural Genomics Consortium (NESG), Ontario Centre for Structural Proteomics (OCSP), Structural Genomics Consortium (SGC)

Function and Biology Details

Reaction catalysed: 
Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-186231 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
OTU domain-containing protein 7A Chain: A
Molecule details ›
Chain: A
Length: 73 amino acids
Theoretical weight: 8.05 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8TE49 (Residues: 11-83; Coverage: 8%)
Gene names: C15orf16, CEZANNE2, OTUD7, OTUD7A
Structure domains: DNA helicase RuvA subunit, C-terminal domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 93%
Refinement method: simulated annealing
Expression system: Escherichia coli

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