2l3j

Solution NMR

The solution structure of the ADAR2 dsRBM-RNA complex reveals a sequence-specific read out of the minor groove

Released:

Function and Biology Details

Reaction catalysed:
Adenine in double-stranded RNA + H(2)O = hypoxanthine in double-stranded RNA + NH(3)
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
PDBe Complex ID:
PDB-CPX-156320 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Double-stranded RNA-specific editase 1 Chain: A
Molecule details ›
Chain: A
Length: 236 amino acids
Theoretical weight: 25.45 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: P51400 (Residues: 74-301; Coverage: 32%)
Gene names: Adarb1, Red1
Sequence domains: Double-stranded RNA binding motif
Structure domains: Double Stranded RNA Binding Domain
RNA (71-MER) Chain: B
Molecule details ›
Chain: B
Length: 71 nucleotides
Theoretical weight: 22.79 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia phage T7
Sequence domains: mir-2985-2 microRNA precursor

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: simulated annealing
Expression systems:
  • Escherichia coli
  • Escherichia phage T7