› 2l4x

Solution NMR

Solution Structure of apo-IscU(WT)

Released:
DOI: 10.2210/pdb2l4x/pdb
PDB entry 2l4x coloured by chain, ensemble of 20 models, front view.
PDB entry 2l4x coloured by chain, ensemble of 20 models, side view.
PDB entry 2l4x coloured by chain, ensemble of 20 models, top view.
Source organism: Escherichia coli K-12
Primary publication:
Three-dimensional structure and determinants of stability of the iron-sulfur cluster scaffold protein IscU from Escherichia coli.
Kim JH, Tonelli M, Kim T, Markley JL
Biochemistry 51 5557-63 (2012)
PMID: 22734684

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142261 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Iron-sulfur cluster assembly scaffold protein IscU Chain: A
Molecule details ›
Chain: A
Length: 128 amino acids
Theoretical weight: 13.87 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P0ACD4 (Residues: 1-128; Coverage: 100%)
Gene names: JW2513, b2529, iscU, nifU, yfhN
Sequence domains: NifU-like N terminal domain
Structure domains: Sufe protein. Chain: A

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: TORSION ANGLE DYNAMICS, simulated annealing
Expression system: Escherichia coli

Quick links

Citations

6 review citations

Mammalian Fe-S proteins: definition of a consensus motif recognized by the co-chaperone HSC20.
Maio et al. (2016)
5 more

14 mentions without citation

Metamorphic protein IscU alternates conformations in the course of its role as the scaffold protein for iron-sulfur cluster biosynthesis and delivery.
Markley et al. (2013)
13 more