› 2lah

Solution NMR

Solution NMR Structure of Mitotic checkpoint serine/threonine-protein kinase BUB1 N-terminal domain from Homo sapiens, Northeast Structural Genomics Consortium Target HR5460A (Methods Development)

Released:
DOI: 10.2210/pdb2lah/pdb
PDB entry 2lah coloured by chain, ensemble of 20 models, front view.
PDB entry 2lah coloured by chain, ensemble of 20 models, side view.
PDB entry 2lah coloured by chain, ensemble of 20 models, top view.
Source organism: Homo sapiens
Entry authors: Liu G, Xiao R, Lee H, Hamilton K, Acton TB, Ciccosanti C, Everett JK, Shastry RT, Huang YJ, Montelione GT, Northeast Structural Genomics Consortium n, Northeast Structural Genomics Consortium (NESG)

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-129061 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Mitotic checkpoint serine/threonine-protein kinase BUB1 Chain: A
Molecule details ›
Chain: A
Length: 160 amino acids
Theoretical weight: 19.21 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O43683 (Residues: 1-150; Coverage: 14%)
Gene names: BUB1, BUB1L
Sequence domains: Mad3/BUB1 homology region 1
Structure domains: Serine Threonine Protein Phosphatase 5, Tetratricopeptide repeat

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 92%
Refinement method: distance geometry, torsion angle dynamics, simulated annealing, molecular dynamics
Expression system: Escherichia coli

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