› 2lex

Solution NMR

Complex of the C-terminal WRKY domain of AtWRKY4 and a W-box DNA

Released:
DOI: 10.2210/pdb2lex/pdb
PDB entry 2lex coloured by chain, ensemble of 20 models, front view.
PDB entry 2lex coloured by chain, ensemble of 20 models, side view.
PDB entry 2lex coloured by chain, ensemble of 20 models, top view.
Source organism: Arabidopsis thaliana
Primary publication:
Structural basis for sequence-specific DNA recognition by an Arabidopsis WRKY transcription factor.
Yamasaki K, Kigawa T, Watanabe S, Inoue M, Yamasaki T, Seki M, Shinozaki K, Yokoyama S
J Biol Chem 287 7683-91 (2012)
PMID: 22219184

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-115539 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Probable WRKY transcription factor 4 Chain: A
Molecule details ›
Chain: A
Length: 78 amino acids
Theoretical weight: 8.59 KDa
Source organism: Arabidopsis thaliana
Expression system: cell free synthesis
UniProt:
  • Canonical: Q9XI90 (Residues: 399-469; Coverage: 14%)
Gene names: At1g13960, F16A14.18, F7A19.5, WRKY4
Sequence domains: WRKY DNA -binding domain
Structure domains: WRKY domain
DNA (5'-D(*CP*G*CP*CP*TP*TP*TP*GP*AP*CP*CP*AP*GP*CP*GP*C)-3') Chain: B
Molecule details ›
Chain: B
Length: 16 nucleotides
Theoretical weight: 4.84 KDa
Source organism: Arabidopsis thaliana
Expression system: Not provided
DNA (5'-D(*GP*CP*GP*C*TP*GP*GP*TP*CP*AP*AP*AP*GP*GP*CP*G)-3') Chain: C
Molecule details ›
Chain: C
Length: 16 nucleotides
Theoretical weight: 4.96 KDa
Source organism: Arabidopsis thaliana
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand ZN 1 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 53%
Refinement method: simulated annealing
Expression system: Not provided

Quick links

Citations

9 review citations

Protein-protein interactions in the regulation of WRKY transcription factors.
Chi et al. (2013)
8 more

11 mentions without citation

Structure, function and networks of transcription factors involved in abiotic stress responses.
Lindemose et al. (2013)
10 more